Highly stable and reusable immobilized formate dehydrogenases: Promising biocatalysts for in situ regeneration of NADH

• Barış Binay,
• Dilek Alagöz,
• Deniz Yildirim,
• Ayhan Çelik and
• S. Seyhan Tükel

Beilstein J. Org. Chem. 2016, 12, 271–277, doi:10.3762/bjoc.12.29

• formate dehydrogenase (FDH) preparations which can be used as effective biocatalysts along with functional oxidoreductases, in which in situ regeneration of NADH is required. For this purpose, Candida methylica FDH was covalently immobilized onto Immobead 150 support (FDHI150), Immobead 150 support

• , FDHIGLU and FDHIALD offer feasible potentials for in situ regeneration of NADH. Keywords: biocatalysis; Candida methylica; formate dehydrogenase; Immobead 150; regeneration of NADH; stabilization; Introduction Dehydrogenases are one of the most promising enzymes in biocatalysis since these enzymes have

• still under discussion, a Schiff base reaction between the carbonyl group of glutaraldehyde and the terminal amino functional group could be expected [25][26]. Candida methylica FDH is a dimeric enzyme [27] and it may be easily inactivated by the dissociation of its subunits depending on reaction